| Gene | Hs6st3 |
| Chromosome | 14 |
| Deletion Start | 119,138,399 |
| Deletion End | 119,139,102 |
| Deletion Size | 704 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,138,425 | 119,138,444 | GGTTCAACAAGTGGCTGCTG |
| TUP | 119,138,446 | 119,138,468 | CGCCGGTGCTCACTCTCCTCTTC |
| TUR | 119,138,504 | 119,138,524 | AAGTTGGTGCATGAGCTGGTG |
| TDF | 119,138,778 | 119,138,798 | TTCGTGCCTCGATTCAACTTC |
| TDP | 119,138,801 | 119,138,823 | CCTCAAGGACCTGACCCGCTTCG |
| TDR | 119,138,837 | 119,138,855 | GATCACATCCCGCCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCGTAGGCCCCGGCTCTG |
| SD | ACATGGCTGAAGGTGACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11012A-B4 | VGB6 | C57BL/6NTac | |
| 11012D-C12 | VGB6 | C57BL/6NTac | |
| 11012E-H3 | VGB6 | C57BL/6NTac | |
