| Gene | Mrpl23 |
| Chromosome | 7 |
| Deletion Start | 142,533,162 |
| Deletion End | 142,540,662 |
| Deletion Size | 7,501 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,534,080 | 142,534,100 | ACCTTGGGAGTCATCTGAAAC |
| TUP | 142,534,102 | 142,534,125 | CTGGTACCTGCTTCTCAGCCCTGA |
| TUR | 142,534,128 | 142,534,148 | AAGCTGAGGGCACCAGAGAAG |
| TDF | 142,540,388 | 142,540,406 | ACACTGGCGGCTCATCATC |
| TDP | 142,540,408 | 142,540,433 | TGTTGTCTGTCTTCCAGAGTCCAGGA |
| TDR | 142,540,453 | 142,540,472 | CCTATCACACCCAGGGACTC |
PCR Primers
| Primer | Sequence |
| SU | GATGGGGCAAATGGGTACAG |
| SD | TGCAATCTGCAACTGTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 437 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 389 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11013B-D1 | VGB6 | C57BL/6NTac | |
| 11013B-F11 | VGB6 | C57BL/6NTac | |
| 11013B-H8 | VGB6 | C57BL/6NTac | |
