| Gene | Mettl22 |
| Chromosome | 16 |
| Deletion Start | 8,473,852 |
| Deletion End | 8,490,186 |
| Deletion Size | 16,353 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,474,393 | 8,474,416 | CGGGTGTACTTCATTCTTCGTGTC |
| TUP | 8,474,420 | 8,474,447 | TAAGTCTTCCTTCACTGTGCACTGCTGT |
| TUR | 8,474,471 | 8,474,490 | CTGCATGTCGGTGGTAGGTC |
| TDF | 8,489,194 | 8,489,212 | CCCTCACAGCAGGGAACTC |
| TDP | 8,489,217 | 8,489,246 | AGCTAGAATCTTGCATCTGCAGTCAAAGCA |
| TDR | 8,489,250 | 8,489,269 | CAAGCTGAGCAGCACCATCC |
PCR Primers
| Primer | Sequence |
| SU | CAGCTTTCTTGGCTGCTTG |
| SD | TTCTCAGTGGAACCCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 452 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 420 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11016A-A7 | VGB6 | C57BL/6NTac | |
| 11016A-B9 | VGB6 | C57BL/6NTac | |
| 11016A-G7 | VGB6 | C57BL/6NTac | |
| 11016A-F9 | VGB6 | C57BL/6NTac | |
