| Gene | Ssxa1 |
| Chromosome | X |
| Deletion Start | 21,118,417 |
| Deletion End | 21,121,439 |
| Deletion Size | 3,023 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,118,799 | 21,118,818 | TCCACCCATCAGGAGCTTTC |
| TUP | 21,118,819 | 21,118,845 | TCTAATTCCCACAAGTTGCGTGGAGGT |
| TUR | 21,118,857 | 21,118,877 | CCTTCAGCCTAGAGCATGTCC |
| TDF | 21,121,002 | 21,121,026 | TGAGGTCCAAGCTCTATACTTTCTG |
| TDP | 21,121,038 | 21,121,066 | CTCTTAAATCCTCTCAGCACCCAGCTCCC |
| TDR | 21,121,089 | 21,121,109 | TCACTGGTCTTCTGAGTCCTG |
PCR Primers
| Primer | Sequence |
| SU | ATCTCCCTCCACAGTACTCC |
| SD | CTGCAAAACATGCATGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 515 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 474 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11018B-F11 | VGB6 | C57BL/6NTac | |
