| Gene | Kif2b |
| Chromosome | 11 |
| Deletion Start | 91,577,452 |
| Deletion End | 91,575,430 |
| Deletion Size | 2,023 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,576,624 | 91,576,644 | ACGACAAGGCATCCAATGAAC |
| TUP | 91,576,598 | 91,576,622 | AGTTTACCAGTTCACCGCCAGGCCT |
| TUR | 91,576,577 | 91,576,597 | TCGGAAGATGGACTCCACAAG |
| TDF | 91,576,169 | 91,576,188 | CCAGGAGCCATGCTGTGTTC |
| TDP | 91,576,144 | 91,576,168 | CAGCTCATTCTCAAGGCTGGAGGGA |
| TDR | 91,576,102 | 91,576,123 | CGTTCCCAGCTAAGTCAACCAG |
PCR Primers
| Primer | Sequence |
| SU | TTAGATCCACCCATGCTCAC |
| SD | CCAGTTGTCAGTATGGATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 407 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11020A-A8 | VGB6 | C57BL/6NTac | |
| 11020A-F1 | VGB6 | C57BL/6NTac | |
| 11020B-E6 | VGB6 | C57BL/6NTac | |
| 11020B-D6 | VGB6 | C57BL/6NTac | |
| 11020B-C12 | VGB6 | C57BL/6NTac | |
| 11020C-G12 | VGB6 | C57BL/6NTac | |
| 11020C-H7 | VGB6 | C57BL/6NTac | |
