| Gene | Sh3bgrl3 |
| Chromosome | 4 |
| Deletion Start | 134,128,660 |
| Deletion End | 134,127,690 |
| Deletion Size | 971 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,128,285 | 134,128,303 | CCTCGCCGAGTCTAGCTTC |
| TUP | 134,128,259 | 134,128,283 | CTGTCATGCACAGAACTTGCCCGTC |
| TUR | 134,128,214 | 134,128,238 | AGGGTCCAAGGATAGACAGGTAAAC |
| TDF | 134,128,067 | 134,128,087 | AGATCAAGTCCCAGCAGAGTG |
| TDP | 134,128,040 | 134,128,063 | TGACCCGCATCCTAGATGGGAAGA |
| TDR | 134,127,988 | 134,128,007 | TCATCTCGAAGGGCGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGCCTTTTAGCCGGTCC |
| SD | TAATTGTCTCTAGGGCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11026B-A12 | VGB6 | C57BL/6NTac | |
| 11026B-A1 | VGB6 | C57BL/6NTac | |
