| Gene | Eif1b |
| Chromosome | 9 |
| Deletion Start | 120,492,814 |
| Deletion End | 120,494,957 |
| Deletion Size | 2,144 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,493,147 | 120,493,167 | AGACCCGTAAGGGAGCTACAG |
| TUP | 120,493,169 | 120,493,191 | ACTTGCCAGGGCTGCAGAGACTA |
| TUR | 120,493,222 | 120,493,241 | GAGACTCCCAGCGTCTTGTC |
| TDF | 120,494,540 | 120,494,558 | TGCCTGCAATGGAACTGTG |
| TDP | 120,494,563 | 120,494,592 | AACACCCTGAGTACGGAGAGGTTATTCAGC |
| TDR | 120,494,628 | 120,494,646 | CGCCACTCACCTCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTGCCCGCAGTGGGCTC |
| SD | AACAAACACTCACAACAAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 447 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11034A-H11 | VGB6 | C57BL/6NTac | |
| 11034A-D1 | VGB6 | C57BL/6NTac | |
| 11034A-D3 | VGB6 | C57BL/6NTac | |
| 11034A-F6 | VGB6 | C57BL/6NTac | |
| 11034A-C11 | VGB6 | C57BL/6NTac | |
