| Gene | Pigc |
| Chromosome | 1 |
| Deletion Start | 161,970,454 |
| Deletion End | 161,971,366 |
| Deletion Size | 913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 161,970,479 | 161,970,499 | CAGAAGTCAAGTGGCAGAAGG |
| TUP | 161,970,501 | 161,970,524 | TTTATATGAGCGGCAGCCGTTCCC |
| TUR | 161,970,535 | 161,970,555 | TTCCAGGAACCTCTGGTCAAC |
| TDF | 161,971,166 | 161,971,190 | CCTTTGGAGGTCTTCTGTCCATTAG |
| TDP | 161,971,191 | 161,971,214 | TGCTGTGGGAGCCATACTCTTTGC |
| TDR | 161,971,230 | 161,971,251 | GTAAGGGCAGAGACAGGAGATG |
PCR Primers
| Primer | Sequence |
| SU | GCCGCATTATGTCACCAGAC |
| SD | CACACATAAATAAGGGTTTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11036B-A6 | VGB6 | C57BL/6NTac | |
| 11036B-F3 | VGB6 | C57BL/6NTac | |
