| Gene | Lenep |
| Chromosome | 3 |
| Deletion Start | 89,402,636 |
| Deletion End | 89,402,382 |
| Deletion Size | 255 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,402,570 | 89,402,589 | AGATGCCCTTCGAGGCACTG |
| TUP | 89,402,540 | 89,402,565 | CCAGGTGCCTGTCATTAAAATGGGCA |
| TUR | 89,402,517 | 89,402,536 | TCGATACATGCCCTCCCAAC |
| TDF | 89,402,472 | 89,402,496 | CATCCTTCTTTGTTGCTGGTGTATC |
| TDP | 89,402,437 | 89,402,462 | CTGGATTAATGGTGGCAGGGAACACC |
| TDR | 89,402,408 | 89,402,426 | TGCAGGTCTTGGTGTCAGG |
PCR Primers
| Primer | Sequence |
| SU | ATCTGAACTGGCAGTGCTCC |
| SD | TTCAAGTACGTGGGTTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11038A-B4 | VGB6 | C57BL/6NTac | |
| 11038A-C9 | VGB6 | C57BL/6NTac | |
| 11038A-D12 | VGB6 | C57BL/6NTac | |
| 11038A-F8 | VGB6 | C57BL/6NTac | |
| 11038A-F11 | VGB6 | C57BL/6NTac | |
