| Gene | Kcng4 |
| Chromosome | 8 |
| Deletion Start | 119,633,632 |
| Deletion End | 119,625,632 |
| Deletion Size | 8,001 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,632,805 | 119,632,826 | GCCTGAAGGCATGTGAAATCTC |
| TUP | 119,632,781 | 119,632,802 | TCCGGGAGACAAGCTGCTCCTA |
| TUR | 119,632,743 | 119,632,765 | CCAGCCAGTAACACTAGGGAAAG |
| TDF | 119,626,138 | 119,626,157 | GGGCACTACGCATCCTCTAC |
| TDP | 119,626,116 | 119,626,136 | TAATGCGCCTGGCTCGCCACT |
| TDR | 119,626,046 | 119,626,068 | AAGAGCATCAGGAGACCAAACTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGAACCTCCTTAGTGTAG |
| SD | GCAGACTCCGTATTTGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | 421 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11043A-A8 | VGB6 | C57BL/6NTac | |
| 11043A-F4 | VGB6 | C57BL/6NTac | |
| 11043A-F7 | VGB6 | C57BL/6NTac | |
| 11043A-F10 | VGB6 | C57BL/6NTac | |
| 11043A-F12 | VGB6 | C57BL/6NTac | |
