| Gene | Ndnl2 |
| Chromosome | 7 |
| Deletion Start | 64,872,915 |
| Deletion End | 64,871,855 |
| Deletion Size | 1,061 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,872,574 | 64,872,596 | TGCAATACGTGTTCGGGTACAAG |
| TUP | 64,872,542 | 64,872,563 | TGGAGCCCAAGAGCCACAGCTA |
| TUR | 64,872,515 | 64,872,535 | ACCGGCTCCAGCATGTTGATG |
| TDF | 64,872,132 | 64,872,149 | GCGAGGCTTTGGCAGATG |
| TDP | 64,872,102 | 64,872,128 | AGAGTAGGGCTAGACCTGCAACTGCTA |
| TDR | 64,872,048 | 64,872,069 | CCAGAGTCCCTCAGACCATAAC |
PCR Primers
| Primer | Sequence |
| SU | TCAATTCTCATTAAAACTGG |
| SD | AACACATCACATAGATTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11045B-D7 | VGB6 | C57BL/6NTac | |
| 11045B-E5 | VGB6 | C57BL/6NTac | |
| 11045A-G3 | VGB6 | C57BL/6NTac | |
| 11045A-A4 | VGB6 | C57BL/6NTac | |
| 11045A-B8 | VGB6 | C57BL/6NTac | |
| 11045A-D5 | VGB6 | C57BL/6NTac | |
