11046 Gemin8 Status: ES cell colonies screened / QC no positives
| Gene | Gemin8 |
| Chromosome | X |
| Deletion Start | 166,178,831 |
| Deletion End | 166,189,531 |
| Deletion Size | 10,721 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 166,179,004 | 166,179,026 | GGACTCAGCGAGCAGATTAGTAG |
| TUP | 166,179,032 | 166,179,056 | AAGGGAAGGAACCCTAGCATGGTCA |
| TUR | 166,179,081 | 166,179,105 | CCTGGCTATCTGTATTAGCTCCAAG |
| TDF | 166,189,147 | 166,189,167 | ATGGCATGTGAGGCGTGGTAG |
| TDP | 166,189,168 | 166,189,193 | CAGGAGTGTCAGCATCCAGCAGATCC |
| TDR | 166,189,223 | 166,189,245 | CCCTGGGAATGGATAGGACTGAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTAGGCTTGGAACCCTTG |
| SD | AATGACACGTGGCTTAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 513 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
