| Gene | Sephs2 |
| Chromosome | 7 |
| Deletion Start | 127,273,916 |
| Deletion End | 127,272,542 |
| Deletion Size | 1,375 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,273,439 | 127,273,458 | GCGCATAGCTTGTGCCAATG |
| TUP | 127,273,414 | 127,273,438 | TGCTCAGTGACCTCTATGCCATGGG |
| TUR | 127,273,365 | 127,273,386 | GCTCTGGCTCACACTGAGTAAC |
| TDF | 127,272,690 | 127,272,711 | AGCTTGGATCGTTGGCATCGTG |
| TDP | 127,272,663 | 127,272,686 | AAGGGAAACCGGACAGCCCGGATC |
| TDR | 127,272,636 | 127,272,659 | AACTTCAATAACGCGAGGCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GACCGGTTGTGCAAGCAGAC |
| SD | AATCTTTACTCCCTGTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 384 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11047A-G8 | VGB6 | C57BL/6NTac | |
| 11047A-B2 | VGB6 | C57BL/6NTac | |
| 11047A-B10 | VGB6 | C57BL/6NTac | |
| 11047A-H6 | VGB6 | C57BL/6NTac | |
| 11047A-H8 | VGB6 | C57BL/6NTac | |
