| Gene | Mbd3l2 |
| Chromosome | 9 |
| Deletion Start | 18,443,583 |
| Deletion End | 18,445,308 |
| Deletion Size | 1,726 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,444,012 | 18,444,035 | GTGTGTGACTTGTTTGATAGAGAC |
| TUP | 18,444,045 | 18,444,064 | CCTATCCTTGATAATCACAT |
| TUR | 18,444,119 | 18,444,138 | TCACAGCATGGCCTAGCTTC |
| TDF | 18,444,753 | 18,444,776 | GGAATGCGGGATGAAACTAATGAC |
| TDP | 18,444,780 | 18,444,809 | AGTGGTGTTAAGGGTCAGCTTACTCCTGGA |
| TDR | 18,444,810 | 18,444,831 | GGGTTTGGACAGAGGTAACTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGATCCACTAGCTCCTG |
| SD | CCCATGGTAGAGAGCAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 347 bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 392 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11048A-H1 | VGB6 | C57BL/6NTac | |
| 11048A-F8 | VGB6 | C57BL/6NTac | |
| 11048C-B4 | VGB6 | C57BL/6NTac | |
| 11048B-B4 | VGB6 | C57BL/6NTac | |
| 11048B-E4 | VGB6 | C57BL/6NTac | |
| 11048B-H2 | VGB6 | C57BL/6NTac | |
| 11048C-B5 | VGB6 | C57BL/6NTac | |
