| Gene | E030025P04Rik |
| Chromosome | 11 |
| Deletion Start | 109,144,057 |
| Deletion End | 109,139,484 |
| Deletion Size | 4,574 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,143,914 | 109,143,932 | CAGCAGGAGGTGGGTCAAC |
| TUP | 109,143,887 | 109,143,912 | TCAGCCAAGACCAGGATGGAAGGGAT |
| TUR | 109,143,843 | 109,143,863 | GCTGTGTCCTCTGTGTCTCTG |
| TDF | 109,140,073 | 109,140,093 | CTGATCCCTGTGATCCTCCAC |
| TDP | 109,140,044 | 109,140,069 | CACAGAAGAAGGCCTAACAGGGAGCA |
| TDR | 109,139,947 | 109,139,967 | CATCCCACTCCTGCTGTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTTGCTGCCCACAGATCAGC |
| SD | ACCAATGGCAGCTTTCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 425 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11049A-A11 | VGB6 | C57BL/6NTac | |
| 11049A-G5 | VGB6 | C57BL/6NTac | |
