| Gene | Rtn4ip1 |
| Chromosome | 10 |
| Deletion Start | 43,902,248 |
| Deletion End | 43,928,456 |
| Deletion Size | 26,209 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,903,171 | 43,903,193 | TTCCTGTATCCTTCTTGCTGTCC |
| TUP | 43,903,196 | 43,903,222 | CGGTTCACAGCTTCTGTTATAGGCCCA |
| TUR | 43,903,228 | 43,903,248 | GAGTTCTGCTCTGTTGCTTGG |
| TDF | 43,928,358 | 43,928,377 | TTGCTCGAAAGATGCCAGTG |
| TDP | 43,928,378 | 43,928,403 | AACTTGTGCGGAAGCTTGGAGCAGAT |
| TDR | 43,928,404 | 43,928,426 | CCCAACGTGTAATCAATGACCTC |
PCR Primers
| Primer | Sequence |
| SU | ACGTGGGGAGCACAGTAGC |
| SD | CTTCTGATAAGCCAGCAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11056B-A9 | VGB6 | C57BL/6NTac | |
| 11056B-B3 | VGB6 | C57BL/6NTac | |
| 11056B-B4 | VGB6 | C57BL/6NTac | |
