| Gene | Klhl25 |
| Chromosome | 7 |
| Deletion Start | 75,865,351 |
| Deletion End | 75,867,208 |
| Deletion Size | 1,858 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,865,446 | 75,865,464 | GGCCCACCTCAACACTCTG |
| TUP | 75,865,465 | 75,865,487 | CGCAAGCACTGCATGTTCACGGA |
| TUR | 75,865,515 | 75,865,533 | CGCACGATGGCAAGGAAAG |
| TDF | 75,866,988 | 75,867,006 | TGGGACCCAGAGGTGTAAG |
| TDP | 75,867,007 | 75,867,033 | ACCCTGGACTGCTATGACCCTACTTCA |
| TDR | 75,867,042 | 75,867,061 | GCACAGTGGTGATGCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GATCTGTGTGATGCCTGGTG |
| SD | CACTTGCTTCTCCTTCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11058A-G5 | VGB6 | C57BL/6NTac | |
| 11058A-F2 | VGB6 | C57BL/6NTac | |
| 11058A-F11 | VGB6 | C57BL/6NTac | |
| 11058B-B3 | VGB6 | C57BL/6NTac | |
| 11058B-F5 | VGB6 | C57BL/6NTac | |
| 11058B-H12 | VGB6 | C57BL/6NTac | |
