| Gene | Mlycd |
| Chromosome | 8 |
| Deletion Start | 119,394,949 |
| Deletion End | 119,410,558 |
| Deletion Size | 15,610 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,395,761 | 119,395,782 | CGAACGTCCATCACCCTAACAG |
| TUP | 119,395,785 | 119,395,811 | TACACGAAATGCACAAACATGGCCAAA |
| TUR | 119,395,834 | 119,395,855 | GAGGACTGGAGGCTTTAGAGAC |
| TDF | 119,410,442 | 119,410,461 | ACCTGGGCTCCAAGAACATC |
| TDP | 119,410,467 | 119,410,489 | TTCCGAGCAGATCCTCAGCCTGG |
| TDR | 119,410,512 | 119,410,533 | GGAAGGCTGTCCCTAGAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CAAGAGGGCGAGTCAGGTG |
| SD | TCCAAAAGGTCTGTGCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11061A-C4 | VGB6 | C57BL/6NTac | |
| 11061A-D2 | VGB6 | C57BL/6NTac | |
| 11061A-D11 | VGB6 | C57BL/6NTac | |
| 11061A-F2 | VGB6 | C57BL/6NTac | |
