| Gene | Spsb4 |
| Chromosome | 9 |
| Deletion Start | 96,996,265 |
| Deletion End | 96,995,580 |
| Deletion Size | 686 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,996,076 | 96,996,094 | GAGGACCGCTCCCTCAATG |
| TUP | 96,996,049 | 96,996,073 | TTTGTCAAGGATGACGACCGCCTCA |
| TUR | 96,996,015 | 96,996,034 | TCTGTGCTCTGGGCTACTGG |
| TDF | 96,995,882 | 96,995,903 | CCCTTTGCACTCTGTGGGTTAC |
| TDP | 96,995,859 | 96,995,881 | ACCGCACTGGTGGGCAGTGACTC |
| TDR | 96,995,803 | 96,995,822 | GGTTCTTGCCGTCGTGGTAG |
PCR Primers
| Primer | Sequence |
| SU | TAGCTAATCACTGGCCTCCG |
| SD | ATAGCTGGTCTCCCACCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11064A-H9 | VGB6 | C57BL/6NTac | |
| 11064A-H10 | VGB6 | C57BL/6NTac | |
| 11064A-C9 | VGB6 | C57BL/6NTac | |
| 11064A-E7 | VGB6 | C57BL/6NTac | |
