| Gene | Sdpr |
| Chromosome | 1 |
| Deletion Start | 51,289,389 |
| Deletion End | 51,301,511 |
| Deletion Size | 12,123 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,289,660 | 51,289,679 | GCATCCAGAACGACCTCACC |
| TUP | 51,289,681 | 51,289,706 | AGCTCTCCAAGTACCAGGCCTCCACC |
| TUR | 51,289,716 | 51,289,734 | TCTCTAGCAGCTTGCTCAC |
| TDF | 51,300,732 | 51,300,752 | GAACAAGTCCCTGGAGGAAAC |
| TDP | 51,300,755 | 51,300,778 | TGCACAACGTGGACCTCTCCTCTG |
| TDR | 51,300,814 | 51,300,836 | TCCATCTTCTCTTCTGCACTATC |
PCR Primers
| Primer | Sequence |
| SU | AGCTCTGCCAAGTTAACATG |
| SD | CAGCTGGTCCTTCAGCTTTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11065A-A12 | VGB6 | C57BL/6NTac | |
| 11065A-B1 | VGB6 | C57BL/6NTac | |
| 11065A-C2 | VGB6 | C57BL/6NTac | |
| 11065A-H7 | VGB6 | C57BL/6NTac | |
