| Gene | Gimap8 |
| Chromosome | 6 |
| Deletion Start | 48,647,439 |
| Deletion End | 48,659,392 |
| Deletion Size | 11,954 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,648,105 | 48,648,124 | GGACCACTTGCACCTTGATG |
| TUP | 48,648,125 | 48,648,151 | TGGGATGAATCACAGAAGCTCCTTCCA |
| TUR | 48,648,189 | 48,648,208 | GTGAGTCCCGCTCTGTCTTC |
| TDF | 48,658,800 | 48,658,819 | GCCTTCTTCTCCCAGCTCAG |
| TDP | 48,658,823 | 48,658,844 | TCAGCCGGTCACCAGTAGCAGC |
| TDR | 48,658,852 | 48,658,872 | GCCAGTCCAGTGTCCTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | GGCCTTTCTGTTACCATCTTTG |
| SD | GCTCTCAGGGAGGAGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11066A-F11 | VGB6 | C57BL/6NTac | |
| 11066A-D8 | VGB6 | C57BL/6NTac | |
| 11066A-E3 | VGB6 | C57BL/6NTac | |
| 11066A-F4 | VGB6 | C57BL/6NTac | |
| 11066A-F1 | VGB6 | C57BL/6NTac | |
| 11066A-C12 | VGB6 | C57BL/6NTac | |
