| Gene | Magea10 |
| Chromosome | X |
| Deletion Start | 72,383,383 |
| Deletion End | 72,382,314 |
| Deletion Size | 1,070 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,383,132 | 72,383,152 | TCCTGCACTGCTATGATTTCC |
| TUP | 72,383,109 | 72,383,131 | TCTCCACGTAGCCAGGTCAGTGC |
| TUR | 72,383,073 | 72,383,090 | TGTGAGGAGCCTGGACTG |
| TDF | 72,382,788 | 72,382,811 | GCTCACTTATGATGGGATGATGAC |
| TDP | 72,382,759 | 72,382,782 | TTCAGGGCATACCAAAGACAGGCC |
| TDR | 72,382,705 | 72,382,726 | CCTCACTGACACAGTTGCCTTC |
PCR Primers
| Primer | Sequence |
| SU | TCATGCACACTTCTGGCAAC |
| SD | TGGTGTCTTGTGACAGATTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11067A-C3 | VGB6 | C57BL/6NTac | |
| 11067B-F6 | VGB6 | C57BL/6NTac | |
