| Gene | Fam113a |
| Chromosome | 2 |
| Deletion Start | 130,423,532 |
| Deletion End | 130,419,036 |
| Deletion Size | 4,497 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,422,635 | 130,422,654 | AGAGCTGCACAACGGGACAC |
| TUP | 130,422,612 | 130,422,634 | AGTACCGCGAGGTCCGCCAGTTC |
| TUR | 130,422,569 | 130,422,590 | TGAGGAAGTAGAAGCGCACAAG |
| TDF | 130,419,572 | 130,419,591 | CCCAGCCTCCTTTGCACATC |
| TDP | 130,419,547 | 130,419,568 | CAGCCTGGCCACCTGTGCTCAT |
| TDR | 130,419,513 | 130,419,535 | AACCCAGAAGGCAGTACCCAATC |
PCR Primers
| Primer | Sequence |
| SU | CTAGATCAGGACATCGCTGC |
| SD | CTAAGCTGAGGCAAGCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11073A-C12 | VGB6 | C57BL/6NTac | |
| 11073A-E6 | VGB6 | C57BL/6NTac | |
