| Gene | B3gat2 |
| Chromosome | 1 |
| Deletion Start | 23,762,638 |
| Deletion End | 23,763,302 |
| Deletion Size | 665 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,762,834 | 23,762,853 | GGAACGAGTCTCGGCCTCAG |
| TUP | 23,762,864 | 23,762,884 | AGCCGCGCTTGCCCACCATCT |
| TUR | 23,762,889 | 23,762,909 | CGACTGTAGGTGGGTGTGATG |
| TDF | 23,763,168 | 23,763,186 | ACCCGGCGTGCTCTTCTTC |
| TDP | 23,763,189 | 23,763,215 | CGACGATGACAACACGTACAGTCTGGA |
| TDR | 23,763,229 | 23,763,247 | ATCGGCGCTGGACCACTAC |
PCR Primers
| Primer | Sequence |
| SU | GCCTGGCTTGCACTCGGCAC |
| SD | TCACCTGTAGGCACTGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 451 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 396 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11075B-A5 | VGB6 | C57BL/6NTac | |
| 11075B-A8 | VGB6 | C57BL/6NTac | |
| 11075B-E9 | VGB6 | C57BL/6NTac | |
| 11075B-D12 | VGB6 | C57BL/6NTac | |
| 11075B-B2 | VGB6 | C57BL/6NTac | |
| 11075B-F10 | VGB6 | C57BL/6NTac | |
