| Gene | 1700012A03Rik |
| Chromosome | 6 |
| Deletion Start | 32,051,618 |
| Deletion End | 32,058,780 |
| Deletion Size | 7,163 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,052,109 | 32,052,129 | CCTCCAGTGTGGCACAGTTAG |
| TUP | 32,052,130 | 32,052,154 | CCTCAGCAGCAAGGAAATGGAAGCA |
| TUR | 32,052,155 | 32,052,176 | TGGCACCAAGGATGGTTTATTC |
| TDF | 32,057,997 | 32,058,017 | TTCCTTCAGGTGGGACTTCAG |
| TDP | 32,058,020 | 32,058,046 | TGTGGGCTGGGAAGTTATCCTACCTGT |
| TDR | 32,058,060 | 32,058,081 | CCAGGACATTGTCTGGTGATTC |
PCR Primers
| Primer | Sequence |
| SU | TGGCTGTGGAGCCAGGAAG |
| SD | ATTGTGGGAAGACATCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 292 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11076C-E3 | VGB6 | C57BL/6NTac | |
| 11076C-F11 | VGB6 | C57BL/6NTac | |
