| Gene | Krtap6-2 |
| Chromosome | 16 |
| Deletion Start | 89,420,074 |
| Deletion End | 89,419,506 |
| Deletion Size | 569 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTGGCTGCTGTGGCTA |
| TUP | 89,419,630 | 89,419,649 | CGGACCATCTTGCTACAGAA |
| TUR | 89,419,603 | 89,419,621 | GGGTGTTCTAACAGCAAGA |
| TDF | 89,419,555 | 89,419,576 | CTGACACTTCTGAAGAAAATGC |
| TDP | 89,419,531 | 89,419,551 | TTTAAAGATCCATGGCCAAGA |
| TDR | 89,419,508 | 89,419,530 | TTGTAGTTTCCTGGATATAGAGC |
PCR Primers
| Primer | Sequence |
| SU | AGGAACATGGTTTGACCGAG |
| SD | TTCAATATAAGCCAAGGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11080B-C12 | VGB6 | C57BL/6NTac | |
| 11080B-G1 | VGB6 | C57BL/6NTac | |
| 11080C-E4 | VGB6 | C57BL/6NTac | |
| 11080C-H1 | VGB6 | C57BL/6NTac | |
