| Gene | Srsf12 |
| Chromosome | 4 |
| Deletion Start | 33,223,555 |
| Deletion End | 33,231,326 |
| Deletion Size | 7,772 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,223,961 | 33,223,982 | TCAAGCAGCAAACAAAGCAATC |
| TUP | 33,223,984 | 33,224,008 | TTGAGACCAGCTGTGTTTGCACAGA |
| TUR | 33,224,009 | 33,224,030 | GACTTGGGTTTGTCTCCAGAAC |
| TDF | 33,230,974 | 33,230,992 | CGCTACCCAGGCAGTCTAC |
| TDP | 33,230,995 | 33,231,016 | CCCTGAGGCAGTCACGGACACC |
| TDR | 33,231,074 | 33,231,097 | GACTGTGATTTCTCCATCGACTTG |
PCR Primers
| Primer | Sequence |
| SU | TACCTAGTGTTAGTGGATTG |
| SD | AATATTTAATACCTAAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | 367 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11082B-F12 | VGB6 | C57BL/6NTac | |
| 11082B-G11 | VGB6 | C57BL/6NTac | |
| 11082A-B6 | VGB6 | C57BL/6NTac | |
