| Gene | Dmrt3 |
| Chromosome | 19 |
| Deletion Start | 25,610,802 |
| Deletion End | 25,623,223 |
| Deletion Size | 12,422 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,610,915 | 25,610,933 | CTGGCTCAAGGGCCACAAG |
| TUP | 25,610,934 | 25,610,958 | CGCTACTGTCGCTTCAAGGACTGCA |
| TUR | 25,611,039 | 25,611,058 | ATGAGGCTCTCCAGGCTCTC |
| TDF | 25,622,757 | 25,622,778 | TGGGCTCCTACCCTATCTCTTC |
| TDP | 25,622,780 | 25,622,800 | TCCAAGTGGTCCGTGGGCTCT |
| TDR | 25,622,835 | 25,622,854 | GACCACGTTGCTGGAGTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGACCTGCGGGTGGAGCCTG |
| SD | CGGCCTTCCCGGGCGTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 144 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 370 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11087A-H3 | VGB6 | C57BL/6NTac | |
| 11087C-B11 | VGB6 | C57BL/6NTac | |
| 11087C-B1 | VGB6 | C57BL/6NTac | |
| 11087C-C11 | VGB6 | C57BL/6NTac | |
