| Gene | Dcaf12l2 |
| Chromosome | X |
| Deletion Start | 44,368,007 |
| Deletion End | 44,366,259 |
| Deletion Size | 1,749 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,367,362 | 44,367,381 | TGATACCGTGGCTGTGAGTG |
| TUP | 44,367,337 | 44,367,357 | CCGTGATGGCACCGTGGCACT |
| TUR | 44,367,295 | 44,367,315 | CAGGGAATGCTGCCATTGAAC |
| TDF | 44,366,949 | 44,366,970 | CAAACCTTTGTGTTCCCGAGAG |
| TDP | 44,366,924 | 44,366,946 | AGGCACAGGTGTGCGTTCTCTGA |
| TDR | 44,366,891 | 44,366,910 | CAGTGCCCACAGTGATGATG |
PCR Primers
| Primer | Sequence |
| SU | CAGCACCCCACGGCCAACAG |
| SD | TAATCTAAAACTTGAAGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 522 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 398 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11089A-E9 | VGB6 | C57BL/6NTac | |
| 11089A-E11 | VGB6 | C57BL/6NTac | |
| 11089A-H6 | VGB6 | C57BL/6NTac | |
