| Gene | Tmco2 |
| Chromosome | 4 |
| Deletion Start | 121,109,154 |
| Deletion End | 121,105,737 |
| Deletion Size | 3,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,108,923 | 121,108,945 | TGCCTTATGGAAACGAAGCATTC |
| TUP | 121,108,874 | 121,108,893 | AATTTAAGTCTTTTGCAGGG |
| TUR | 121,108,825 | 121,108,846 | GCCAGCCTAAAGTACCACTCTG |
| TDF | 121,105,838 | 121,105,858 | GGATAATCGTCGCCCGGAAAC |
| TDP | 121,105,811 | 121,105,833 | TTCGAAGCGGGACTGCTCCTCTG |
| TDR | 121,105,749 | 121,105,770 | TGTGGAAGTGAACCCTGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TATTGGTTGCTGTATCAGAC |
| SD | CTATCTTGCATTACCATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 378 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11090A-G1 | VGB6 | C57BL/6NTac | |
| 11090A-B2 | VGB6 | C57BL/6NTac | |
