| Gene | Unc13d |
| Chromosome | 11 |
| Deletion Start | 116,077,558 |
| Deletion End | 116,062,651 |
| Deletion Size | 14,908 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,076,799 | 116,076,818 | GGGACGCCGTGTCTTTCTAC |
| TUP | 116,076,772 | 116,076,796 | TGCTCCTAGGTCCAGGTTCAGTCCC |
| TUR | 116,076,727 | 116,076,751 | ACACTCTCCCAACATCTCCTCTTAC |
| TDF | 116,063,269 | 116,063,288 | CACTCAGGCAGGTGGATCTG |
| TDP | 116,063,247 | 116,063,268 | TCAGCCTGAGGCCAAACCGAAC |
| TDR | 116,063,215 | 116,063,235 | TCTGACTGAGACAGGGTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CTCTCCCCAGAGCCTCCGTG |
| SD | CTGGCAGTTCCAAAAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 392 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 360 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11093B-C4 | VGB6 | C57BL/6NTac | |
| 11093B-F3 | VGB6 | C57BL/6NTac | |
