| Gene | Hyal5 |
| Chromosome | 6 |
| Deletion Start | 24,876,133 |
| Deletion End | 24,891,777 |
| Deletion Size | 15,645 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,876,540 | 24,876,558 | CAATGTGGGCCTGGCTGTC |
| TUP | 24,876,560 | 24,876,585 | TTGATTGGGAAGAATGGCGGCCTACC |
| TUR | 24,876,627 | 24,876,647 | TGCTGGACCAACTCAATAGAC |
| TDF | 24,891,258 | 24,891,279 | ACTTGAAGACCATCCTGAATCC |
| TDP | 24,891,283 | 24,891,308 | TATCATCAACGTCACCCTTGCAGCCA |
| TDR | 24,891,313 | 24,891,332 | GGCAAAGGGTTTGGCTACAC |
PCR Primers
| Primer | Sequence |
| SU | TAAACACAGTAGGAGATTAC |
| SD | TAGAGCACTTCAGAAATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 438 bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 429 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11100A-D4 | VGB6 | C57BL/6NTac | |
| 11100A-C3 | VGB6 | C57BL/6NTac | |
| 11100A-A12 | VGB6 | C57BL/6NTac | |
