11101 Armcx3 Status: Cancelled
| Gene | Armcx3 |
| Chromosome | X |
| Deletion Start | 134,758,585 |
| Deletion End | 134,759,729 |
| Deletion Size | 1,163 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,758,653 | 134,758,677 | TGCTATTGCATTTACAGATTGACCC |
| TUP | 134,758,685 | 134,758,714 | AAAGCAGAACAAGGAGAAAATGGCTGAGGG |
| TUR | 134,758,841 | 134,758,861 | GCTCTGGTCCCTGCTTCAGTG |
| TDF | 134,759,619 | 134,759,643 | TGTGCTGATAAAGTTCTAGGAATCG |
| TDP | 134,759,646 | 134,759,669 | AGCCGTCATGATTTCCAGGTGCGA |
| TDR | 134,759,693 | 134,759,716 | GGGAACATCCTTTCAGTCAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTACTTTCTTGCCCTACTGG |
| SD | GATGAATTGATTCGCTGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 209 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
