| Gene | Cyp2s1 |
| Chromosome | 7 |
| Deletion Start | 25,816,499 |
| Deletion End | 25,803,251 |
| Deletion Size | 13,249 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,815,987 | 25,816,007 | GAACAGGACCCAGAGGAGTAG |
| TUP | 25,815,962 | 25,815,981 | CCAGGTCGCAGGGACCCACA |
| TUR | 25,815,929 | 25,815,950 | GCTTTGGTCTCAGCTCACTAAC |
| TDF | 25,803,332 | 25,803,351 | TGGTGACACAAGCCGAACTC |
| TDP | 25,803,306 | 25,803,328 | CTTGTGATCCTCCCGCCTCGGTG |
| TDR | 25,803,269 | 25,803,287 | TCATGGTGGCGCATACCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTACTATGCCCCACCTAGG |
| SD | TCCATGGGAGTAGACAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 360 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11104A-A12 | VGB6 | C57BL/6NTac | |
| 11104A-E5 | VGB6 | C57BL/6NTac | |
