| Gene | Pold4 |
| Chromosome | 19 |
| Deletion Start | 4,232,076 |
| Deletion End | 4,233,241 |
| Deletion Size | 1,166 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,232,404 | 4,232,423 | AAGTCCAGGGTAGGGCAAAG |
| TUP | 4,232,426 | 4,232,448 | CTGGATGTGCCTGAGGAGCCACC |
| TUR | 4,232,492 | 4,232,510 | GCAGCTCCAGCTCTGTTTC |
| TDF | 4,232,712 | 4,232,732 | TCTCCTGCCTATCCTGTCTTC |
| TDP | 4,232,734 | 4,232,759 | TTTCCTGATGGCAGGTATCACAAGGC |
| TDR | 4,232,770 | 4,232,788 | CATCTGCTCTGCCCGACTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGCCGATTGCCATTGGC |
| SD | GGTTATCTGCCGACATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 394 bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11107A-A1 | VGB6 | C57BL/6NTac | |
| 11107A-B3 | VGB6 | C57BL/6NTac | |
| 11107A-C5 | VGB6 | C57BL/6NTac | |
| 11107A-D8 | VGB6 | C57BL/6NTac | |
| 11107A-F9 | VGB6 | C57BL/6NTac | |
