| Gene | Prrt3 |
| Chromosome | 6 |
| Deletion Start | 113,498,256 |
| Deletion End | 113,494,291 |
| Deletion Size | 3,966 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,497,784 | 113,497,804 | ACTCTCGGCAGCCATACTTTC |
| TUP | 113,497,762 | 113,497,783 | CACTGCCTCCCAGAAGCCTGGA |
| TUR | 113,497,714 | 113,497,736 | GACCATTATTAGCTCCTCGTTCC |
| TDF | 113,494,508 | 113,494,528 | CAGCTCCCTGGACAGTTTCTC |
| TDP | 113,494,483 | 113,494,507 | CAAGGGCTCGCTGAAGATCAGCTGG |
| TDR | 113,494,420 | 113,494,437 | GGTGCTGGGCAGTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TCTCGGAAAGCCAGGAATAG |
| SD | TCTGCTGTGAGGAGAGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 413 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 511 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11108A-A6 | VGB6 | C57BL/6NTac | |
| 11108A-B1 | VGB6 | C57BL/6NTac | |
| 11108A-C11 | VGB6 | C57BL/6NTac | |
| 11108A-F11 | VGB6 | C57BL/6NTac | |
| 11108A-D5 | VGB6 | C57BL/6NTac | |
| 11108A-C7 | VGB6 | C57BL/6NTac | |
