| Gene | Nudt18 |
| Chromosome | 14 |
| Deletion Start | 70,577,934 |
| Deletion End | 70,579,929 |
| Deletion Size | 1,996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,578,562 | 70,578,581 | GACTTGACACAGGCGGTTTG |
| TUP | 70,578,588 | 70,578,614 | CCATGGGAACAAGAGCAAGACAGTGGC |
| TUR | 70,578,625 | 70,578,646 | GGTTGCCACCACTCCTATACTG |
| TDF | 70,579,480 | 70,579,501 | CACCCTCTCATTCTGCCTCAAG |
| TDP | 70,579,508 | 70,579,530 | CCTGCAGTGTGGTCTGCCAACGG |
| TDR | 70,579,550 | 70,579,568 | ACCCACACCGACTGGACTG |
PCR Primers
| Primer | Sequence |
| SU | AGGGGGCAGAGGGAGGATC |
| SD | CCAAAGTGGACAAAGATGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11110B-B12 | VGB6 | C57BL/6NTac | |
| 11110B-D12 | VGB6 | C57BL/6NTac | |
