| Gene | Cdr2l |
| Chromosome | 11 |
| Deletion Start | 115,382,307 |
| Deletion End | 115,394,272 |
| Deletion Size | 11,966 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,383,078 | 115,383,100 | GGGAGTTAGAAGCTGGTGATGAG |
| TUP | 115,383,111 | 115,383,136 | TGACATCACTGAGAGGTGGAGCCGAC |
| TUR | 115,383,158 | 115,383,176 | CCTTGGCCTTGACCCTGAC |
| TDF | 115,393,876 | 115,393,895 | GCAGGCGCGGTATGTCTATC |
| TDP | 115,393,906 | 115,393,927 | TGGATGAGCAGTACCACGCGCT |
| TDR | 115,393,934 | 115,393,955 | GCTCAGTAGCTCCTCGTATTTC |
PCR Primers
| Primer | Sequence |
| SU | CACCCGGCGTCCCCAGTTCG |
| SD | AGCAATCTGAGGGTGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 372 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11117A-C12 | VGB6 | C57BL/6NTac | |
| 11117B-B4 | VGB6 | C57BL/6NTac | |
| 11117B-E3 | VGB6 | C57BL/6NTac | |
| 11117B-E5 | VGB6 | C57BL/6NTac | |
| 11117B-E10 | VGB6 | C57BL/6NTac | |
