| Gene | Slc35d3 |
| Chromosome | 10 |
| Deletion Start | 19,851,019 |
| Deletion End | 19,848,783 |
| Deletion Size | 2,237 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,850,533 | 19,850,551 | CAACCCACCCGGTAGAGAC |
| TUP | 19,850,509 | 19,850,530 | CTGGGACCACTCGGTGCAACGC |
| TUR | 19,850,470 | 19,850,491 | CAAAGCTCCCTGCCTCAGTATC |
| TDF | 19,849,359 | 19,849,381 | CACTGCACTGTACCTACATCAAC |
| TDP | 19,849,336 | 19,849,358 | TCTGCTGTGACCACCAGCTTCGT |
| TDR | 19,849,305 | 19,849,326 | CGTGATAGTGGCGATGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CATCAGGGATCCTCACCTAC |
| SD | ACAAGCCATCTTTGGAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11122B-B6 | VGB6 | C57BL/6NTac | |
| 11122B-C7 | VGB6 | C57BL/6NTac | |
| 11122B-C12 | VGB6 | C57BL/6NTac | |
| 11122B-F10 | VGB6 | C57BL/6NTac | |
| 11122B-D9 | VGB6 | C57BL/6NTac | |
