| Gene | Dusp21 |
| Chromosome | X |
| Deletion Start | 18,145,952 |
| Deletion End | 18,146,533 |
| Deletion Size | 582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,146,088 | 18,146,111 | TCACCACGATCATCAATGTCTCTG |
| TUP | 18,146,126 | 18,146,155 | ACGTTCTTTGAAGACATTCAGTACGTGCAA |
| TUR | 18,146,162 | 18,146,185 | GTAAGAATTAGGAGCATCGCTCAC |
| TDF | 18,146,338 | 18,146,358 | TGCACACACATGGACCAAGAC |
| TDP | 18,146,359 | 18,146,379 | CTGCCGCCCCATCATCCGTCC |
| TDR | 18,146,383 | 18,146,405 | TCAGTTGTTCCCAAAAGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGGTGAAGGTAGACTGTG |
| SD | GCCTTCTGTCAGGTCCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 382 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11124A-F6 | VGB6 | C57BL/6NTac | |
| 11124A-F10 | VGB6 | C57BL/6NTac | |
