| Gene | Dbndd1 |
| Chromosome | 8 |
| Deletion Start | 123,515,260 |
| Deletion End | 123,506,625 |
| Deletion Size | 8,636 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,514,659 | 123,514,680 | TTGGAAGGGAAGGTGTGAATGG |
| TUP | 123,514,637 | 123,514,658 | TGTGCTCCTTCCTCGGCCTCTC |
| TUR | 123,514,590 | 123,514,609 | GCAGACCCTGCGGTAAGATG |
| TDF | 123,507,408 | 123,507,428 | CCTCCAGCCTGCATGTGTATG |
| TDP | 123,507,382 | 123,507,406 | TGAGTCAGGAGATGGCCCGTGACAT |
| TDR | 123,507,347 | 123,507,366 | GGAGTTGGGCTTCCACTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACCTGTGCTGCTGGCTGCT |
| SD | AAGACCCAGACCACTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11129B-G6 | VGB6 | C57BL/6NTac | |
| 11129B-B2 | VGB6 | C57BL/6NTac | |
