| Gene | Hsfy2 |
| Chromosome | 1 |
| Deletion Start | 56,637,373 |
| Deletion End | 56,636,133 |
| Deletion Size | 1,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,637,189 | 56,637,210 | TGATAAAGAGGCCACCGCATAC |
| TUP | 56,637,165 | 56,637,188 | CCTCTGTGCCTCAGAGCCAGATGA |
| TUR | 56,637,074 | 56,637,095 | GTCCTCATCCCACCAAATAGAC |
| TDF | 56,636,260 | 56,636,281 | GGCGAACCTGAGTTACCGATAC |
| TDP | 56,636,229 | 56,636,258 | CAAGGAAAGAGTAGCATCTACCACGTTGGC |
| TDR | 56,636,193 | 56,636,215 | TGGCCTCAAGAAGATGAGTGATG |
PCR Primers
| Primer | Sequence |
| SU | AAGTGTCTTTAGGGACGACAC |
| SD | AGAGGGATTTGGAGCCCCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 388 bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11130A-G10 | VGB6 | C57BL/6NTac | |
| 11130A-D8 | VGB6 | C57BL/6NTac | |
