11132   Clrn3     Status: ES cell colonies screened / QC positives

GeneClrn3
Chromosome7
Deletion Start135,528,500
Deletion End135,513,934
Deletion Size14,567
Genome Build38
MGI IDMGI:2142022
NCBI Gene ID212070
BAC ID220B3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,528,459135,528,481TGATGTTCCTGTCTGGCTTTCTC
TUP135,528,432135,528,457CAAGCCTGGGCTCAGTTGTTGTCATC
TUR135,528,396135,528,415GCTGGTGATCCATGCCTGAG
TDF135,514,207135,514,228GCTCCATCTTCTTTGCAGCATC
TDP135,514,178135,514,204TTGTGTTTCTGGCCATGGTTCTGTTCG
TDR135,514,156135,514,174TGGTTGGACTCGGCATTCC

PCR Primers

PrimerSequence
SUTGGCTGCAACCATAACAACC
SDCAAATGAGAAAGGCCTGCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev370 bp-++
TUFTUR86 bp++-
TDFTDR73 bp++-
NeoFwdSD357 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11132A-A11VGB6C57BL/6NTac
11132A-E11VGB6C57BL/6NTac
11132A-G5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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