| Gene | Hus1b |
| Chromosome | 13 |
| Deletion Start | 30,947,671 |
| Deletion End | 30,946,803 |
| Deletion Size | 869 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,947,492 | 30,947,509 | ATGCGGCGCGATGTCTTC |
| TUP | 30,947,468 | 30,947,489 | CCACTTCTGCATGGAGGGTGCC |
| TUR | 30,947,369 | 30,947,388 | GAGCTGGAGCTTCAGGGATG |
| TDF | 30,947,206 | 30,947,228 | GGGCTCTGATGTGAGTGTTTATC |
| TDP | 30,947,179 | 30,947,205 | TGCCAGCTCTGAAGACCTTGAAGAACA |
| TDR | 30,947,142 | 30,947,163 | AGCACATGACTGCCCACGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GTGGCCTCAGGTGTAAGTGG |
| SD | TCTCGGAATCTGTGGTGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 363 bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 489 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11134A-A4 | VGB6 | C57BL/6NTac | |
| 11134A-H10 | VGB6 | C57BL/6NTac | |
| 11134A-A11 | VGB6 | C57BL/6NTac | |
| 11134A-D12 | VGB6 | C57BL/6NTac | |
| 11134A-D4 | VGB6 | C57BL/6NTac | |
