| Gene | Il28b |
| Chromosome | 7 |
| Deletion Start | 28,522,821 |
| Deletion End | 28,524,338 |
| Deletion Size | 1,518 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,523,477 | 28,523,497 | CAGGAGAAGAGGCTGCTTGAG |
| TUP | 28,523,498 | 28,523,522 | AAGGACATGAGGTGCAGTTCCCACC |
| TUR | 28,523,525 | 28,523,542 | GTCCCAGGCCCTGGAGAT |
| TDF | 28,523,969 | 28,523,990 | CTGGCTCCTCCTCAGTGTTCTC |
| TDP | 28,523,991 | 28,524,015 | CAGCCACTTGGGCTTCTCCTGTGTC |
| TDR | 28,524,042 | 28,524,064 | TGAAGCTGTGTCTGTGGATAGAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGCAACAGGCCACAGGG |
| SD | CCTGTAGACTTGAAGTTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11135B-A6 | VGB6 | C57BL/6NTac | |
| 11135B-B8 | VGB6 | C57BL/6NTac | |
| 11135B-H11 | VGB6 | C57BL/6NTac | |
