| Gene | Atp6v1e2 |
| Chromosome | 17 |
| Deletion Start | 86,944,965 |
| Deletion End | 86,944,196 |
| Deletion Size | 770 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,944,735 | 86,944,757 | AGCTGTCCACCATGAGAAACCAG |
| TUP | 86,944,711 | 86,944,732 | TCGCATCACGGTCCTGAGAGCC |
| TUR | 86,944,674 | 86,944,696 | TGGCATCCTTGAGCAATTCTAAG |
| TDF | 86,944,365 | 86,944,384 | CCTGGAAAGTCGGCTGAATC |
| TDP | 86,944,344 | 86,944,364 | TCGCGGCCATGCAGAAGATGC |
| TDR | 86,944,279 | 86,944,300 | CCCAGAGCCTCATGTAAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCATTGCCTTCCTACCTG |
| SD | TTTCTGAGCGTGAACCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11137C-D4 | VGB6 | C57BL/6NTac | |
| 11137C-G2 | VGB6 | C57BL/6NTac | |
| 11137C-C5 | VGB6 | C57BL/6NTac | |
| 11137C-D12 | VGB6 | C57BL/6NTac | |
