11144 Bag5 Status: ES cell colonies screened / QC no positives
| Gene | Bag5 |
| Chromosome | 12 |
| Deletion Start | 111,711,384 |
| Deletion End | 111,710,020 |
| Deletion Size | 1,365 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,710,796 | 111,710,815 | CGAGGTGAACAAGGCCAGAG |
| TUP | 111,710,770 | 111,710,793 | ACGCTGATCGCACTTCTGATGGGC |
| TUR | 111,710,725 | 111,710,743 | TGACAGCACGCAGGATAAG |
| TDF | 111,710,175 | 111,710,196 | CTGGAGGAGCTACTGACCAAAC |
| TDP | 111,710,149 | 111,710,174 | AGTTGCTGGCTCTGGATGCCGTTGAC |
| TDR | 111,710,116 | 111,710,134 | CCTGGCAGCCTTACACTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCACATGTGTTCTGGAATTC |
| SD | TCAACCCTGGAATTCCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
