| Gene | Stx19 |
| Chromosome | 16 |
| Deletion Start | 62,821,826 |
| Deletion End | 62,822,738 |
| Deletion Size | 913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,822,190 | 62,822,213 | CATCATCAGTGGTCACAAGGATAC |
| TUP | 62,822,214 | 62,822,241 | TGAAGTCTCAGTATGCTGCGATGTTCCG |
| TUR | 62,822,244 | 62,822,265 | AGAAACATGGTTTGCTGGAAGC |
| TDF | 62,822,417 | 62,822,441 | GAAACCAGTATCACTAAAGCACAGC |
| TDP | 62,822,442 | 62,822,471 | TCTCGGAAATTGAACAGAGACACAAGGAAC |
| TDR | 62,822,483 | 62,822,504 | GGAGGTCCTTCACTTGGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CACAGCAGGAGCAACCATTC |
| SD | AAGAGGCATTCTCGTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11147A-A9 | VGB6 | C57BL/6NTac | |
| 11147A-F4 | VGB6 | C57BL/6NTac | |
| 11147A-G3 | VGB6 | C57BL/6NTac | |
| 11147A-G12 | VGB6 | C57BL/6NTac | |
| 11147A-F6 | VGB6 | C57BL/6NTac | |
