| Gene | Ppapdc2 |
| Chromosome | 19 |
| Deletion Start | 28,964,004 |
| Deletion End | 28,964,883 |
| Deletion Size | 880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,964,257 | 28,964,276 | ACGCCCGCATGAACTTGAAC |
| TUP | 28,964,280 | 28,964,299 | TCCTTCCTGGGCATCGCCCT |
| TUR | 28,964,319 | 28,964,337 | TCTTGGACAGCCACAGGTC |
| TDF | 28,964,647 | 28,964,666 | CCCTGGTGTCGCGGTTTATC |
| TDP | 28,964,669 | 28,964,693 | AAACCACCTGGTACTGGCCATTCCA |
| TDR | 28,964,712 | 28,964,730 | CCAAGACGAAGGCCCACAG |
PCR Primers
| Primer | Sequence |
| SU | GCTGTTTATCCTAACCTCCC |
| SD | AATAGCAATCATACAGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 366 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11148A-B10 | VGB6 | C57BL/6NTac | |
