| Gene | Gimap5 |
| Chromosome | 6 |
| Deletion Start | 48,750,463 |
| Deletion End | 48,753,470 |
| Deletion Size | 3,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,750,736 | 48,750,758 | ACTTGCACATACACACTCCTCTG |
| TUP | 48,750,760 | 48,750,789 | AACACTGTGGAAGCTTGTCTTTGACACTCA |
| TUR | 48,750,794 | 48,750,813 | GTTCCAGGCCCTTCTAACTC |
| TDF | 48,753,309 | 48,753,330 | TTTGCACAGTCAAGTCCTGCTG |
| TDP | 48,753,332 | 48,753,355 | AGCTCCCACACTGCAGCATGTGCT |
| TDR | 48,753,371 | 48,753,394 | GAAAGTGGTGAGAAGAGTCAAACC |
PCR Primers
| Primer | Sequence |
| SU | CTCCCAGGGAAAGTATGCTG |
| SD | AGTGCTTCATGCACACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11149A-F3 | VGB6 | C57BL/6NTac | |
| 11149A-E7 | VGB6 | C57BL/6NTac | |
| 11149A-H6 | VGB6 | C57BL/6NTac | |
| 11149A-H9 | VGB6 | C57BL/6NTac | |
| 11149A-A8 | VGB6 | C57BL/6NTac | |
